Journal: Biomedicines

Article Title: Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

doi: 10.3390/biomedicines8070200

Figure Lengend Snippet: Flowchart of the experimental design. Normal fibroblast (NF), normal keratinocyte (NK), keloid fibroblast (KF), keloid keratinocyte (KK).

Article Snippet: Briefly, samples were placed in keratinocytes growth media (KGM, EpigrowTM, human epidermal keratinocyte complete culture media kit, Merck, Millipore, MA, USA) where the tissue was cut into strips of 3–4 mm 2 to allow dispase to infiltrate the tissue.

Techniques:

Journal: Biomedicines

Article Title: Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

doi: 10.3390/biomedicines8070200

Figure Lengend Snippet: Keloid keratinocytes significantly increase collagen production by normal skin fibroblasts. Keloid keratinocytes and normal skin keratinocytes were co-cultured with normal fibroblasts from patient 1 (NF1). Both normal keratinocytes and 4/6 keloid keratinocyte samples significantly increased COLI produced by NF1. *** = p -value < 0.001.

Article Snippet: Briefly, samples were placed in keratinocytes growth media (KGM, EpigrowTM, human epidermal keratinocyte complete culture media kit, Merck, Millipore, MA, USA) where the tissue was cut into strips of 3–4 mm 2 to allow dispase to infiltrate the tissue.

Techniques: Cell Culture, Produced

Journal: Biomedicines

Article Title: Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

doi: 10.3390/biomedicines8070200

Figure Lengend Snippet: Comparison of the COL1A production evaluated by immunohistochemistry staining of normal fibroblasts culture from patient 1 (NF1) ( A ), when co-cultured with normal keratinocytes (NK1) ( B ), or keloid keratinocytes (Patient 1 keloid keratinocytes (KK)) ( C ).

Article Snippet: Briefly, samples were placed in keratinocytes growth media (KGM, EpigrowTM, human epidermal keratinocyte complete culture media kit, Merck, Millipore, MA, USA) where the tissue was cut into strips of 3–4 mm 2 to allow dispase to infiltrate the tissue.

Techniques: Comparison, Immunohistochemistry, Staining, Cell Culture

Journal: Biomedicines

Article Title: Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

doi: 10.3390/biomedicines8070200

Figure Lengend Snippet: Keloid keratinocytes significantly increase collagen production by normal skin fibroblasts. Keloid keratinocytes and normal skin keratinocytes were co-cultured with normal fibroblasts from patient 2 (NF2). 5/6 keloid keratinocyte samples increased NF2 COLI production. No effect was seen with normal keratinocytes or with 1 keloid keratinocyte sample. ** = p -value < 0.01.

Article Snippet: Briefly, samples were placed in keratinocytes growth media (KGM, EpigrowTM, human epidermal keratinocyte complete culture media kit, Merck, Millipore, MA, USA) where the tissue was cut into strips of 3–4 mm 2 to allow dispase to infiltrate the tissue.

Techniques: Cell Culture

Journal: Biomedicines

Article Title: Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

doi: 10.3390/biomedicines8070200

Figure Lengend Snippet: Comparison of the COL1A production evaluated by immunohistochemistry staining of normal fibroblasts culture from patient 2 (NF2) ( A ), when co-cultured with normal keratinocytes (NK1) ( B ), or keloid keratinocytes (Patient 1 keloid keratinocytes (KK)) ( C ).

Article Snippet: Briefly, samples were placed in keratinocytes growth media (KGM, EpigrowTM, human epidermal keratinocyte complete culture media kit, Merck, Millipore, MA, USA) where the tissue was cut into strips of 3–4 mm 2 to allow dispase to infiltrate the tissue.

Techniques: Comparison, Immunohistochemistry, Staining, Cell Culture

Journal: Biomedicines

Article Title: Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

doi: 10.3390/biomedicines8070200

Figure Lengend Snippet: Keloid keratinocytes significantly decrease collagen production by keloid fibroblasts. Keloid fibroblasts from patients 4, 5 and 6 were cultured with matched keloid keratinocytes or normal keratinocytes (NK1). Keloid keratinocytes significantly reduced COLI produced by the matched keloid fibroblasts ( A – C ). * = p -value < 0.05, *** = p -value < 0.001.

Article Snippet: Briefly, samples were placed in keratinocytes growth media (KGM, EpigrowTM, human epidermal keratinocyte complete culture media kit, Merck, Millipore, MA, USA) where the tissue was cut into strips of 3–4 mm 2 to allow dispase to infiltrate the tissue.

Techniques: Cell Culture, Produced

Journal: Biomedicines

Article Title: Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

doi: 10.3390/biomedicines8070200

Figure Lengend Snippet: Comparison of the COL1A production evaluated by immunohistochemistry staining of keloid fibroblasts culture from patient 4 (KF) ( A ), when co-cultured with normal keratinocytes (NK1) ( B ), or keloid keratinocytes (Patient 4 keloid keratinocytes (KK)) ( C ).

Article Snippet: Briefly, samples were placed in keratinocytes growth media (KGM, EpigrowTM, human epidermal keratinocyte complete culture media kit, Merck, Millipore, MA, USA) where the tissue was cut into strips of 3–4 mm 2 to allow dispase to infiltrate the tissue.

Techniques: Comparison, Immunohistochemistry, Staining, Cell Culture